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cdh2 thermo fisher scientific pa5  (Thermo Fisher)


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    Thermo Fisher cdh2 thermo fisher scientific pa5
    Cdh2 Thermo Fisher Scientific Pa5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdh2+thermo+fisher+scientific+pa5/pmc10033729__41467_2023_37161_MOESM1_ESM-103-297-298
    Average 86 stars, based on 1 article reviews
    cdh2 thermo fisher scientific pa5 - by Bioz Stars, 2026-09
    86/100 stars

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    Expressing:

    Article Title: YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells
    Article Snippet: 139 HPV Status Tobacco Status Neoplasm Histologic Grade Tumor Stage Code (AJCC) Neoplasm Disease Lymph Node Stage (AJCC) YBX1 YBX1_PS102 YBX1 YBX1 YBX1_PS102 mRNA expression z-scores Protein expression z-scores 16% 4% Genetic Alteration Missense Mutation (unknown significance) Amplification mRNA High mRNA Low Protein High Protein Low No alterations HPV Status No Yes Tobacco Status No Yes Neoplasm Histologic Grade G1 G2 G3 G4 GX Tumour Stage Code (AJCC) T1 T2 T3 T4A TX No data Neoplasm Disease Lymph Node Stage (AJCC) N0 N1 N2 N2A N2B N2C N3 NX No data Expression Heatmap -3 3 Figure S8: Expression of pYBX1 and PDPN in patient HNC samples 50 100 150 PDPN 0 pYBX1 PDPN ROI % P os iti ve Ce lls 0 50 100 150 pYBX1 % Po si tiv e Ce lls pYBX1 PDPN ROI pYBX1 PDPN Patient No. (n=135) Differentiation Grading Well 51 Moderate 60 Poor 23 Ki67 0 13 1 67 2 37 3 6 4 12 p16 Negative 111 Positive 24 p53 Negative 68 Positive 48 Overexpression 19 A B C D 140 Antibody Company Catalogue No. Clone Dilution Assay Actin Abcam ab8229 1:3000 WB CDH1 Cell Signaling Technology CST3195 24E10 1:1000, 1:200 WB, IHC PDPN Abcam ab128994 EPR7072 1:1000, 1:200 WB, IHC EGFR Cell Signaling Technology CST4267 D38B1 1:1000, 1:50 WB, IHC p110 Cell Signaling Technology CST4249 C73F8 1:1000 WB p85 Cell Signaling Technology CST4257 19H8 1:1000 WB pAKT (Ser473) Cell Signaling Technology CST9271 1:1000 WB AKT Cell Signaling Technology CST9272 1:1000 WB pRPS6 (Ser240/244) Cell Signaling Technology CST2215 1:1000 WB RPS6 Cell Signaling Technology CST2217 5G10 1:1000 WB pEIF4E (Ser209) Abcam ab76256 EP2151Y 1:1000, 1:100 WB, IHC EIF4E Cell Signaling Technology CST9742 1:1000, 1:50 WB, IHC pEIF4EBP1 Cell Signaling Technology CST9451 1:1000 WB EIF4EBP1 Cell Signaling Technology CST9644 53H11 1:1000 WB CDH2 Thermo Fisher Scientific PA5-17526 1:1000 WB TWIST1 Thermo Fisher Scientific 711565 1:1000 WB Tubulin Cell Signaling Technology CST3873 DM1A 1:3000 WB pYBX1 (Ser102) Cell Signaling Technology CST2900 C34A2 1:1000, 1:200, 1:200 WB, IHC, IF YBX1 Cell Signaling Technology CST4202 1:1000, 1:50, 1:50 WB, IHC, IF H2A Cell Signaling Technology CST12349 D6O3A 1:1000 WB GFP Santa Cruz Biotechnology sc-9996 B-2 1:50 IF MYC tag Cell Signaling Technology CST47029 9B11 1:1000; 1:50 WB, FACS Goat-anti-rabbit 647 Abcam ab150079 1:500 IF Goat-anti-rabbit 488 Abcam ab150077 1:500 IF Goat-anti-mouse 488 Abcam ab150113 1:200 IF Goat-anti-rabbit-HRP Bio-rad 1706515 1:1000 WB Goat-anti-mouse-HRP Bio-rad 1706516 1:1000 WB Supplementary Table_Antibodies list

    Mutagenesis:

    Article Title: YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells
    Article Snippet: 139 HPV Status Tobacco Status Neoplasm Histologic Grade Tumor Stage Code (AJCC) Neoplasm Disease Lymph Node Stage (AJCC) YBX1 YBX1_PS102 YBX1 YBX1 YBX1_PS102 mRNA expression z-scores Protein expression z-scores 16% 4% Genetic Alteration Missense Mutation (unknown significance) Amplification mRNA High mRNA Low Protein High Protein Low No alterations HPV Status No Yes Tobacco Status No Yes Neoplasm Histologic Grade G1 G2 G3 G4 GX Tumour Stage Code (AJCC) T1 T2 T3 T4A TX No data Neoplasm Disease Lymph Node Stage (AJCC) N0 N1 N2 N2A N2B N2C N3 NX No data Expression Heatmap -3 3 Figure S8: Expression of pYBX1 and PDPN in patient HNC samples 50 100 150 PDPN 0 pYBX1 PDPN ROI % P os iti ve Ce lls 0 50 100 150 pYBX1 % Po si tiv e Ce lls pYBX1 PDPN ROI pYBX1 PDPN Patient No. (n=135) Differentiation Grading Well 51 Moderate 60 Poor 23 Ki67 0 13 1 67 2 37 3 6 4 12 p16 Negative 111 Positive 24 p53 Negative 68 Positive 48 Overexpression 19 A B C D 140 Antibody Company Catalogue No. Clone Dilution Assay Actin Abcam ab8229 1:3000 WB CDH1 Cell Signaling Technology CST3195 24E10 1:1000, 1:200 WB, IHC PDPN Abcam ab128994 EPR7072 1:1000, 1:200 WB, IHC EGFR Cell Signaling Technology CST4267 D38B1 1:1000, 1:50 WB, IHC p110 Cell Signaling Technology CST4249 C73F8 1:1000 WB p85 Cell Signaling Technology CST4257 19H8 1:1000 WB pAKT (Ser473) Cell Signaling Technology CST9271 1:1000 WB AKT Cell Signaling Technology CST9272 1:1000 WB pRPS6 (Ser240/244) Cell Signaling Technology CST2215 1:1000 WB RPS6 Cell Signaling Technology CST2217 5G10 1:1000 WB pEIF4E (Ser209) Abcam ab76256 EP2151Y 1:1000, 1:100 WB, IHC EIF4E Cell Signaling Technology CST9742 1:1000, 1:50 WB, IHC pEIF4EBP1 Cell Signaling Technology CST9451 1:1000 WB EIF4EBP1 Cell Signaling Technology CST9644 53H11 1:1000 WB CDH2 Thermo Fisher Scientific PA5-17526 1:1000 WB TWIST1 Thermo Fisher Scientific 711565 1:1000 WB Tubulin Cell Signaling Technology CST3873 DM1A 1:3000 WB pYBX1 (Ser102) Cell Signaling Technology CST2900 C34A2 1:1000, 1:200, 1:200 WB, IHC, IF YBX1 Cell Signaling Technology CST4202 1:1000, 1:50, 1:50 WB, IHC, IF H2A Cell Signaling Technology CST12349 D6O3A 1:1000 WB GFP Santa Cruz Biotechnology sc-9996 B-2 1:50 IF MYC tag Cell Signaling Technology CST47029 9B11 1:1000; 1:50 WB, FACS Goat-anti-rabbit 647 Abcam ab150079 1:500 IF Goat-anti-rabbit 488 Abcam ab150077 1:500 IF Goat-anti-mouse 488 Abcam ab150113 1:200 IF Goat-anti-rabbit-HRP Bio-rad 1706515 1:1000 WB Goat-anti-mouse-HRP Bio-rad 1706516 1:1000 WB Supplementary Table_Antibodies list

    Amplification:

    Article Title: YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells
    Article Snippet: 139 HPV Status Tobacco Status Neoplasm Histologic Grade Tumor Stage Code (AJCC) Neoplasm Disease Lymph Node Stage (AJCC) YBX1 YBX1_PS102 YBX1 YBX1 YBX1_PS102 mRNA expression z-scores Protein expression z-scores 16% 4% Genetic Alteration Missense Mutation (unknown significance) Amplification mRNA High mRNA Low Protein High Protein Low No alterations HPV Status No Yes Tobacco Status No Yes Neoplasm Histologic Grade G1 G2 G3 G4 GX Tumour Stage Code (AJCC) T1 T2 T3 T4A TX No data Neoplasm Disease Lymph Node Stage (AJCC) N0 N1 N2 N2A N2B N2C N3 NX No data Expression Heatmap -3 3 Figure S8: Expression of pYBX1 and PDPN in patient HNC samples 50 100 150 PDPN 0 pYBX1 PDPN ROI % P os iti ve Ce lls 0 50 100 150 pYBX1 % Po si tiv e Ce lls pYBX1 PDPN ROI pYBX1 PDPN Patient No. (n=135) Differentiation Grading Well 51 Moderate 60 Poor 23 Ki67 0 13 1 67 2 37 3 6 4 12 p16 Negative 111 Positive 24 p53 Negative 68 Positive 48 Overexpression 19 A B C D 140 Antibody Company Catalogue No. Clone Dilution Assay Actin Abcam ab8229 1:3000 WB CDH1 Cell Signaling Technology CST3195 24E10 1:1000, 1:200 WB, IHC PDPN Abcam ab128994 EPR7072 1:1000, 1:200 WB, IHC EGFR Cell Signaling Technology CST4267 D38B1 1:1000, 1:50 WB, IHC p110 Cell Signaling Technology CST4249 C73F8 1:1000 WB p85 Cell Signaling Technology CST4257 19H8 1:1000 WB pAKT (Ser473) Cell Signaling Technology CST9271 1:1000 WB AKT Cell Signaling Technology CST9272 1:1000 WB pRPS6 (Ser240/244) Cell Signaling Technology CST2215 1:1000 WB RPS6 Cell Signaling Technology CST2217 5G10 1:1000 WB pEIF4E (Ser209) Abcam ab76256 EP2151Y 1:1000, 1:100 WB, IHC EIF4E Cell Signaling Technology CST9742 1:1000, 1:50 WB, IHC pEIF4EBP1 Cell Signaling Technology CST9451 1:1000 WB EIF4EBP1 Cell Signaling Technology CST9644 53H11 1:1000 WB CDH2 Thermo Fisher Scientific PA5-17526 1:1000 WB TWIST1 Thermo Fisher Scientific 711565 1:1000 WB Tubulin Cell Signaling Technology CST3873 DM1A 1:3000 WB pYBX1 (Ser102) Cell Signaling Technology CST2900 C34A2 1:1000, 1:200, 1:200 WB, IHC, IF YBX1 Cell Signaling Technology CST4202 1:1000, 1:50, 1:50 WB, IHC, IF H2A Cell Signaling Technology CST12349 D6O3A 1:1000 WB GFP Santa Cruz Biotechnology sc-9996 B-2 1:50 IF MYC tag Cell Signaling Technology CST47029 9B11 1:1000; 1:50 WB, FACS Goat-anti-rabbit 647 Abcam ab150079 1:500 IF Goat-anti-rabbit 488 Abcam ab150077 1:500 IF Goat-anti-mouse 488 Abcam ab150113 1:200 IF Goat-anti-rabbit-HRP Bio-rad 1706515 1:1000 WB Goat-anti-mouse-HRP Bio-rad 1706516 1:1000 WB Supplementary Table_Antibodies list

    Over Expression:

    Article Title: YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells
    Article Snippet: 139 HPV Status Tobacco Status Neoplasm Histologic Grade Tumor Stage Code (AJCC) Neoplasm Disease Lymph Node Stage (AJCC) YBX1 YBX1_PS102 YBX1 YBX1 YBX1_PS102 mRNA expression z-scores Protein expression z-scores 16% 4% Genetic Alteration Missense Mutation (unknown significance) Amplification mRNA High mRNA Low Protein High Protein Low No alterations HPV Status No Yes Tobacco Status No Yes Neoplasm Histologic Grade G1 G2 G3 G4 GX Tumour Stage Code (AJCC) T1 T2 T3 T4A TX No data Neoplasm Disease Lymph Node Stage (AJCC) N0 N1 N2 N2A N2B N2C N3 NX No data Expression Heatmap -3 3 Figure S8: Expression of pYBX1 and PDPN in patient HNC samples 50 100 150 PDPN 0 pYBX1 PDPN ROI % P os iti ve Ce lls 0 50 100 150 pYBX1 % Po si tiv e Ce lls pYBX1 PDPN ROI pYBX1 PDPN Patient No. (n=135) Differentiation Grading Well 51 Moderate 60 Poor 23 Ki67 0 13 1 67 2 37 3 6 4 12 p16 Negative 111 Positive 24 p53 Negative 68 Positive 48 Overexpression 19 A B C D 140 Antibody Company Catalogue No. Clone Dilution Assay Actin Abcam ab8229 1:3000 WB CDH1 Cell Signaling Technology CST3195 24E10 1:1000, 1:200 WB, IHC PDPN Abcam ab128994 EPR7072 1:1000, 1:200 WB, IHC EGFR Cell Signaling Technology CST4267 D38B1 1:1000, 1:50 WB, IHC p110 Cell Signaling Technology CST4249 C73F8 1:1000 WB p85 Cell Signaling Technology CST4257 19H8 1:1000 WB pAKT (Ser473) Cell Signaling Technology CST9271 1:1000 WB AKT Cell Signaling Technology CST9272 1:1000 WB pRPS6 (Ser240/244) Cell Signaling Technology CST2215 1:1000 WB RPS6 Cell Signaling Technology CST2217 5G10 1:1000 WB pEIF4E (Ser209) Abcam ab76256 EP2151Y 1:1000, 1:100 WB, IHC EIF4E Cell Signaling Technology CST9742 1:1000, 1:50 WB, IHC pEIF4EBP1 Cell Signaling Technology CST9451 1:1000 WB EIF4EBP1 Cell Signaling Technology CST9644 53H11 1:1000 WB CDH2 Thermo Fisher Scientific PA5-17526 1:1000 WB TWIST1 Thermo Fisher Scientific 711565 1:1000 WB Tubulin Cell Signaling Technology CST3873 DM1A 1:3000 WB pYBX1 (Ser102) Cell Signaling Technology CST2900 C34A2 1:1000, 1:200, 1:200 WB, IHC, IF YBX1 Cell Signaling Technology CST4202 1:1000, 1:50, 1:50 WB, IHC, IF H2A Cell Signaling Technology CST12349 D6O3A 1:1000 WB GFP Santa Cruz Biotechnology sc-9996 B-2 1:50 IF MYC tag Cell Signaling Technology CST47029 9B11 1:1000; 1:50 WB, FACS Goat-anti-rabbit 647 Abcam ab150079 1:500 IF Goat-anti-rabbit 488 Abcam ab150077 1:500 IF Goat-anti-mouse 488 Abcam ab150113 1:200 IF Goat-anti-rabbit-HRP Bio-rad 1706515 1:1000 WB Goat-anti-mouse-HRP Bio-rad 1706516 1:1000 WB Supplementary Table_Antibodies list

    Dilution Assay:

    Article Title: YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells
    Article Snippet: 139 HPV Status Tobacco Status Neoplasm Histologic Grade Tumor Stage Code (AJCC) Neoplasm Disease Lymph Node Stage (AJCC) YBX1 YBX1_PS102 YBX1 YBX1 YBX1_PS102 mRNA expression z-scores Protein expression z-scores 16% 4% Genetic Alteration Missense Mutation (unknown significance) Amplification mRNA High mRNA Low Protein High Protein Low No alterations HPV Status No Yes Tobacco Status No Yes Neoplasm Histologic Grade G1 G2 G3 G4 GX Tumour Stage Code (AJCC) T1 T2 T3 T4A TX No data Neoplasm Disease Lymph Node Stage (AJCC) N0 N1 N2 N2A N2B N2C N3 NX No data Expression Heatmap -3 3 Figure S8: Expression of pYBX1 and PDPN in patient HNC samples 50 100 150 PDPN 0 pYBX1 PDPN ROI % P os iti ve Ce lls 0 50 100 150 pYBX1 % Po si tiv e Ce lls pYBX1 PDPN ROI pYBX1 PDPN Patient No. (n=135) Differentiation Grading Well 51 Moderate 60 Poor 23 Ki67 0 13 1 67 2 37 3 6 4 12 p16 Negative 111 Positive 24 p53 Negative 68 Positive 48 Overexpression 19 A B C D 140 Antibody Company Catalogue No. Clone Dilution Assay Actin Abcam ab8229 1:3000 WB CDH1 Cell Signaling Technology CST3195 24E10 1:1000, 1:200 WB, IHC PDPN Abcam ab128994 EPR7072 1:1000, 1:200 WB, IHC EGFR Cell Signaling Technology CST4267 D38B1 1:1000, 1:50 WB, IHC p110 Cell Signaling Technology CST4249 C73F8 1:1000 WB p85 Cell Signaling Technology CST4257 19H8 1:1000 WB pAKT (Ser473) Cell Signaling Technology CST9271 1:1000 WB AKT Cell Signaling Technology CST9272 1:1000 WB pRPS6 (Ser240/244) Cell Signaling Technology CST2215 1:1000 WB RPS6 Cell Signaling Technology CST2217 5G10 1:1000 WB pEIF4E (Ser209) Abcam ab76256 EP2151Y 1:1000, 1:100 WB, IHC EIF4E Cell Signaling Technology CST9742 1:1000, 1:50 WB, IHC pEIF4EBP1 Cell Signaling Technology CST9451 1:1000 WB EIF4EBP1 Cell Signaling Technology CST9644 53H11 1:1000 WB CDH2 Thermo Fisher Scientific PA5-17526 1:1000 WB TWIST1 Thermo Fisher Scientific 711565 1:1000 WB Tubulin Cell Signaling Technology CST3873 DM1A 1:3000 WB pYBX1 (Ser102) Cell Signaling Technology CST2900 C34A2 1:1000, 1:200, 1:200 WB, IHC, IF YBX1 Cell Signaling Technology CST4202 1:1000, 1:50, 1:50 WB, IHC, IF H2A Cell Signaling Technology CST12349 D6O3A 1:1000 WB GFP Santa Cruz Biotechnology sc-9996 B-2 1:50 IF MYC tag Cell Signaling Technology CST47029 9B11 1:1000; 1:50 WB, FACS Goat-anti-rabbit 647 Abcam ab150079 1:500 IF Goat-anti-rabbit 488 Abcam ab150077 1:500 IF Goat-anti-mouse 488 Abcam ab150113 1:200 IF Goat-anti-rabbit-HRP Bio-rad 1706515 1:1000 WB Goat-anti-mouse-HRP Bio-rad 1706516 1:1000 WB Supplementary Table_Antibodies list

    Western Blot:

    Article Title: YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells
    Article Snippet: 139 HPV Status Tobacco Status Neoplasm Histologic Grade Tumor Stage Code (AJCC) Neoplasm Disease Lymph Node Stage (AJCC) YBX1 YBX1_PS102 YBX1 YBX1 YBX1_PS102 mRNA expression z-scores Protein expression z-scores 16% 4% Genetic Alteration Missense Mutation (unknown significance) Amplification mRNA High mRNA Low Protein High Protein Low No alterations HPV Status No Yes Tobacco Status No Yes Neoplasm Histologic Grade G1 G2 G3 G4 GX Tumour Stage Code (AJCC) T1 T2 T3 T4A TX No data Neoplasm Disease Lymph Node Stage (AJCC) N0 N1 N2 N2A N2B N2C N3 NX No data Expression Heatmap -3 3 Figure S8: Expression of pYBX1 and PDPN in patient HNC samples 50 100 150 PDPN 0 pYBX1 PDPN ROI % P os iti ve Ce lls 0 50 100 150 pYBX1 % Po si tiv e Ce lls pYBX1 PDPN ROI pYBX1 PDPN Patient No. (n=135) Differentiation Grading Well 51 Moderate 60 Poor 23 Ki67 0 13 1 67 2 37 3 6 4 12 p16 Negative 111 Positive 24 p53 Negative 68 Positive 48 Overexpression 19 A B C D 140 Antibody Company Catalogue No. Clone Dilution Assay Actin Abcam ab8229 1:3000 WB CDH1 Cell Signaling Technology CST3195 24E10 1:1000, 1:200 WB, IHC PDPN Abcam ab128994 EPR7072 1:1000, 1:200 WB, IHC EGFR Cell Signaling Technology CST4267 D38B1 1:1000, 1:50 WB, IHC p110 Cell Signaling Technology CST4249 C73F8 1:1000 WB p85 Cell Signaling Technology CST4257 19H8 1:1000 WB pAKT (Ser473) Cell Signaling Technology CST9271 1:1000 WB AKT Cell Signaling Technology CST9272 1:1000 WB pRPS6 (Ser240/244) Cell Signaling Technology CST2215 1:1000 WB RPS6 Cell Signaling Technology CST2217 5G10 1:1000 WB pEIF4E (Ser209) Abcam ab76256 EP2151Y 1:1000, 1:100 WB, IHC EIF4E Cell Signaling Technology CST9742 1:1000, 1:50 WB, IHC pEIF4EBP1 Cell Signaling Technology CST9451 1:1000 WB EIF4EBP1 Cell Signaling Technology CST9644 53H11 1:1000 WB CDH2 Thermo Fisher Scientific PA5-17526 1:1000 WB TWIST1 Thermo Fisher Scientific 711565 1:1000 WB Tubulin Cell Signaling Technology CST3873 DM1A 1:3000 WB pYBX1 (Ser102) Cell Signaling Technology CST2900 C34A2 1:1000, 1:200, 1:200 WB, IHC, IF YBX1 Cell Signaling Technology CST4202 1:1000, 1:50, 1:50 WB, IHC, IF H2A Cell Signaling Technology CST12349 D6O3A 1:1000 WB GFP Santa Cruz Biotechnology sc-9996 B-2 1:50 IF MYC tag Cell Signaling Technology CST47029 9B11 1:1000; 1:50 WB, FACS Goat-anti-rabbit 647 Abcam ab150079 1:500 IF Goat-anti-rabbit 488 Abcam ab150077 1:500 IF Goat-anti-mouse 488 Abcam ab150113 1:200 IF Goat-anti-rabbit-HRP Bio-rad 1706515 1:1000 WB Goat-anti-mouse-HRP Bio-rad 1706516 1:1000 WB Supplementary Table_Antibodies list

    Immunohistochemistry:

    Article Title: YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells
    Article Snippet: 139 HPV Status Tobacco Status Neoplasm Histologic Grade Tumor Stage Code (AJCC) Neoplasm Disease Lymph Node Stage (AJCC) YBX1 YBX1_PS102 YBX1 YBX1 YBX1_PS102 mRNA expression z-scores Protein expression z-scores 16% 4% Genetic Alteration Missense Mutation (unknown significance) Amplification mRNA High mRNA Low Protein High Protein Low No alterations HPV Status No Yes Tobacco Status No Yes Neoplasm Histologic Grade G1 G2 G3 G4 GX Tumour Stage Code (AJCC) T1 T2 T3 T4A TX No data Neoplasm Disease Lymph Node Stage (AJCC) N0 N1 N2 N2A N2B N2C N3 NX No data Expression Heatmap -3 3 Figure S8: Expression of pYBX1 and PDPN in patient HNC samples 50 100 150 PDPN 0 pYBX1 PDPN ROI % P os iti ve Ce lls 0 50 100 150 pYBX1 % Po si tiv e Ce lls pYBX1 PDPN ROI pYBX1 PDPN Patient No. (n=135) Differentiation Grading Well 51 Moderate 60 Poor 23 Ki67 0 13 1 67 2 37 3 6 4 12 p16 Negative 111 Positive 24 p53 Negative 68 Positive 48 Overexpression 19 A B C D 140 Antibody Company Catalogue No. Clone Dilution Assay Actin Abcam ab8229 1:3000 WB CDH1 Cell Signaling Technology CST3195 24E10 1:1000, 1:200 WB, IHC PDPN Abcam ab128994 EPR7072 1:1000, 1:200 WB, IHC EGFR Cell Signaling Technology CST4267 D38B1 1:1000, 1:50 WB, IHC p110 Cell Signaling Technology CST4249 C73F8 1:1000 WB p85 Cell Signaling Technology CST4257 19H8 1:1000 WB pAKT (Ser473) Cell Signaling Technology CST9271 1:1000 WB AKT Cell Signaling Technology CST9272 1:1000 WB pRPS6 (Ser240/244) Cell Signaling Technology CST2215 1:1000 WB RPS6 Cell Signaling Technology CST2217 5G10 1:1000 WB pEIF4E (Ser209) Abcam ab76256 EP2151Y 1:1000, 1:100 WB, IHC EIF4E Cell Signaling Technology CST9742 1:1000, 1:50 WB, IHC pEIF4EBP1 Cell Signaling Technology CST9451 1:1000 WB EIF4EBP1 Cell Signaling Technology CST9644 53H11 1:1000 WB CDH2 Thermo Fisher Scientific PA5-17526 1:1000 WB TWIST1 Thermo Fisher Scientific 711565 1:1000 WB Tubulin Cell Signaling Technology CST3873 DM1A 1:3000 WB pYBX1 (Ser102) Cell Signaling Technology CST2900 C34A2 1:1000, 1:200, 1:200 WB, IHC, IF YBX1 Cell Signaling Technology CST4202 1:1000, 1:50, 1:50 WB, IHC, IF H2A Cell Signaling Technology CST12349 D6O3A 1:1000 WB GFP Santa Cruz Biotechnology sc-9996 B-2 1:50 IF MYC tag Cell Signaling Technology CST47029 9B11 1:1000; 1:50 WB, FACS Goat-anti-rabbit 647 Abcam ab150079 1:500 IF Goat-anti-rabbit 488 Abcam ab150077 1:500 IF Goat-anti-mouse 488 Abcam ab150113 1:200 IF Goat-anti-rabbit-HRP Bio-rad 1706515 1:1000 WB Goat-anti-mouse-HRP Bio-rad 1706516 1:1000 WB Supplementary Table_Antibodies list

    FACS:

    Article Title: YBX1 integration of oncogenic PI3K/mTOR signalling regulates the fitness of malignant epithelial cells
    Article Snippet: 139 HPV Status Tobacco Status Neoplasm Histologic Grade Tumor Stage Code (AJCC) Neoplasm Disease Lymph Node Stage (AJCC) YBX1 YBX1_PS102 YBX1 YBX1 YBX1_PS102 mRNA expression z-scores Protein expression z-scores 16% 4% Genetic Alteration Missense Mutation (unknown significance) Amplification mRNA High mRNA Low Protein High Protein Low No alterations HPV Status No Yes Tobacco Status No Yes Neoplasm Histologic Grade G1 G2 G3 G4 GX Tumour Stage Code (AJCC) T1 T2 T3 T4A TX No data Neoplasm Disease Lymph Node Stage (AJCC) N0 N1 N2 N2A N2B N2C N3 NX No data Expression Heatmap -3 3 Figure S8: Expression of pYBX1 and PDPN in patient HNC samples 50 100 150 PDPN 0 pYBX1 PDPN ROI % P os iti ve Ce lls 0 50 100 150 pYBX1 % Po si tiv e Ce lls pYBX1 PDPN ROI pYBX1 PDPN Patient No. (n=135) Differentiation Grading Well 51 Moderate 60 Poor 23 Ki67 0 13 1 67 2 37 3 6 4 12 p16 Negative 111 Positive 24 p53 Negative 68 Positive 48 Overexpression 19 A B C D 140 Antibody Company Catalogue No. Clone Dilution Assay Actin Abcam ab8229 1:3000 WB CDH1 Cell Signaling Technology CST3195 24E10 1:1000, 1:200 WB, IHC PDPN Abcam ab128994 EPR7072 1:1000, 1:200 WB, IHC EGFR Cell Signaling Technology CST4267 D38B1 1:1000, 1:50 WB, IHC p110 Cell Signaling Technology CST4249 C73F8 1:1000 WB p85 Cell Signaling Technology CST4257 19H8 1:1000 WB pAKT (Ser473) Cell Signaling Technology CST9271 1:1000 WB AKT Cell Signaling Technology CST9272 1:1000 WB pRPS6 (Ser240/244) Cell Signaling Technology CST2215 1:1000 WB RPS6 Cell Signaling Technology CST2217 5G10 1:1000 WB pEIF4E (Ser209) Abcam ab76256 EP2151Y 1:1000, 1:100 WB, IHC EIF4E Cell Signaling Technology CST9742 1:1000, 1:50 WB, IHC pEIF4EBP1 Cell Signaling Technology CST9451 1:1000 WB EIF4EBP1 Cell Signaling Technology CST9644 53H11 1:1000 WB CDH2 Thermo Fisher Scientific PA5-17526 1:1000 WB TWIST1 Thermo Fisher Scientific 711565 1:1000 WB Tubulin Cell Signaling Technology CST3873 DM1A 1:3000 WB pYBX1 (Ser102) Cell Signaling Technology CST2900 C34A2 1:1000, 1:200, 1:200 WB, IHC, IF YBX1 Cell Signaling Technology CST4202 1:1000, 1:50, 1:50 WB, IHC, IF H2A Cell Signaling Technology CST12349 D6O3A 1:1000 WB GFP Santa Cruz Biotechnology sc-9996 B-2 1:50 IF MYC tag Cell Signaling Technology CST47029 9B11 1:1000; 1:50 WB, FACS Goat-anti-rabbit 647 Abcam ab150079 1:500 IF Goat-anti-rabbit 488 Abcam ab150077 1:500 IF Goat-anti-mouse 488 Abcam ab150113 1:200 IF Goat-anti-rabbit-HRP Bio-rad 1706515 1:1000 WB Goat-anti-mouse-HRP Bio-rad 1706516 1:1000 WB Supplementary Table_Antibodies list



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